Biological control of Fusarium solani f. sp. phaseoli the causal agent of root rot of bean using Bacillus subtilis CA32 and Trichoderma harzianum RU01

Saman Abeysinghe

Abstract


Root rot, caused by Fusarium solani f. sp. phaseoli, is one of the main root diseases impacting production of common bean in Sri Lanka. Rhizobacteria were screened in dual Petri plate assays to select antagonistic strains against F. solani f. sp. phaseoli. B. subtilis CA32 effectively antagonized the pathogen. T. harzianum RU01 also showed the antagonistic activity. The efficacy of the B. subtilis CA32 and the T. harzianum RU01 were tested in greenhouse pot experiments against F. solani f. sp. phaseoli. Seed bacterization with B. subtilis CA32 and T. harzianum RU01 significantly protected bean seedlings from F. solani f. sp. phaseoli compared to the untreated control plants. Plant protection was more pronounced in T. harzianum RU01 treated plants than bacterized plants. Enhanced root growth was observed only T. harzianum RU01 treated plants, suggesting that the biotic modifications of the mycorrhizosphere as a result of colonization with T. harzianum RU01.

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Abeysinghe, S. The effect of mode of application of Bacillus subtilis CA32r on control of Sclerotium rolfsii on Capsicum annuum. Archives of Pytopathology & Plant Protection (in press).

Askew, D. G., Laing, M. D. (1993) An adapted selective medium for the quantitative isolation of Trichoderma species. Plant Pathology 42:686-690.

Booth C. 1977. Fusarium: Laboratory Guide to the Identification of the Major Species. Kew, UK: Commonwealth Mycological Institute.

Burke, D. W., and Hall, R. Compendium of bean diseases. St. Paul, Minnesota, USA, APS Press, 1991, 9-10pp.

Filion, M., St-Arnaud, M., Jabaji-Hare, S. H. (2003) Quantification of Fusarium solani f. sp. phaseoli in mycorrhizal bean plants and surrounding mycorrhizosphere soil using realtime polymerase chain reaction and direct isolations on selective media. Phytopathology

:229-235.

Gams, W., Bisset, J. (1998) Morphology and identification of Trichoderma. In: Kubicek, C. P., Herman, C. E. (Eds.), Trichoderma and Gliocladium: Basic Biology, Taxonomy and Genetics. Taylor and Francis, London, UK, pp. 3-34.

Handelsman, J. & Stabb, E. C. (1996) Biocontrol of soilborne plant pathogens. The Plant Cell 8:1855-1869.

Hanson, L. E. & Howell, C. R. (2004) Elicitors of plant defense responses from biocontrol strains of Trichoderma virens. Phytopathology 94:171-176.

Spadaro, D., & Gullino, M. L. (2005) Improving the efficacy of biocontrol agents against soilborne pathogens. Crop Protection 24:601-613.

Steyaert, J. M., Ridgway, H. J., Elad, Y., Stewart, A. (2003) Genetic basis of mycoparasitism: a mechanism of biological control by species of Trichoderma. New Zealand Journal of Crop and Horticultural Science 31:281-291.

Vinale, F., Marra, R., Scala, F., Ghisalberti, E. L., Lorito, M., Sivasithamparam, K. (2006) Major secondary metabolites produced by two commercial Trichoderma strains active against different phytopathogens. Letters in Applied Microbiology 43:143-148.


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